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The pET28b-ACP-G2-Amy-Histag, pET30a-His 6 -MBP-BCCP, pET28b-MBP-TEV and pBAD34-AcpS constructs were generated in our laboratory previously for expression of the fusion protein ACP-Amy and MBP-BCCP, TEV and holo-(acyl carrier protein) synthase AcpS, respectively (Chen et al
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Protected by her asylum status, Shakur lived openly in Cuba
